diff --git a/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts b/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
index ebe2e74da5..aede682866 100644
--- a/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
+++ b/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
@@ -1,11 +1,13 @@
 import { getStore } from 'app/store/nanostores/store';
 import type { RootState } from 'app/store/store';
 import {
+  NEGATIVE_CONDITIONING,
   NEGATIVE_CONDITIONING_COLLECT,
   POSITIVE_CONDITIONING,
   POSITIVE_CONDITIONING_COLLECT,
-  PROMPT_REGION_COND_PREFIX,
   PROMPT_REGION_MASK_PREFIX,
+  PROMPT_REGION_NEGATIVE_COND_PREFIX,
+  PROMPT_REGION_POSITIVE_COND_PREFIX,
 } from 'features/nodes/util/graph/constants';
 import { getRegionalPromptLayerBlobs } from 'features/regionalPrompts/util/getLayerBlobs';
 import { size } from 'lodash-es';
@@ -116,34 +118,55 @@ export const addRegionalPromptsToGraph = async (state: RootState, graph: NonNull
     // Create the conditioning nodes for each region - different handling for SDXL
 
     // TODO: negative prompt
-    const regionalCondNodeId = `${PROMPT_REGION_COND_PREFIX}_${layerId}`;
+    const regionalPositiveCondNodeId = `${PROMPT_REGION_POSITIVE_COND_PREFIX}_${layerId}`;
+    const regionalNegativeCondNodeId = `${PROMPT_REGION_NEGATIVE_COND_PREFIX}_${layerId}`;
 
     if (isSDXL) {
-      graph.nodes[regionalCondNodeId] = {
+      graph.nodes[regionalPositiveCondNodeId] = {
         type: 'sdxl_compel_prompt',
-        id: regionalCondNodeId,
-        prompt: layer.prompt,
+        id: regionalPositiveCondNodeId,
+        prompt: layer.positivePrompt,
+      };
+      graph.nodes[regionalNegativeCondNodeId] = {
+        type: 'sdxl_compel_prompt',
+        id: regionalNegativeCondNodeId,
+        prompt: layer.negativePrompt,
       };
     } else {
-      graph.nodes[regionalCondNodeId] = {
-        type: 'compel',
-        id: regionalCondNodeId,
-        prompt: layer.prompt,
-      };
+      // TODO: non sdxl
+      // graph.nodes[regionalCondNodeId] = {
+      //   type: 'compel',
+      //   id: regionalCondNodeId,
+      //   prompt: layer.prompt,
+      // };
     }
     graph.edges.push({
       source: { node_id: id, field: 'mask' },
-      destination: { node_id: regionalCondNodeId, field: 'mask' },
+      destination: { node_id: regionalPositiveCondNodeId, field: 'mask' },
     });
     graph.edges.push({
-      source: { node_id: regionalCondNodeId, field: 'conditioning' },
+      source: { node_id: id, field: 'mask' },
+      destination: { node_id: regionalNegativeCondNodeId, field: 'mask' },
+    });
+    graph.edges.push({
+      source: { node_id: regionalPositiveCondNodeId, field: 'conditioning' },
       destination: { node_id: posCondCollectNode.id, field: 'item' },
     });
+    graph.edges.push({
+      source: { node_id: regionalNegativeCondNodeId, field: 'conditioning' },
+      destination: { node_id: negCondCollectNode.id, field: 'item' },
+    });
     for (const edge of graph.edges) {
       if (edge.destination.node_id === POSITIVE_CONDITIONING && edge.destination.field !== 'prompt') {
         graph.edges.push({
           source: edge.source,
-          destination: { node_id: regionalCondNodeId, field: edge.destination.field },
+          destination: { node_id: regionalPositiveCondNodeId, field: edge.destination.field },
+        });
+      }
+      if (edge.destination.node_id === NEGATIVE_CONDITIONING && edge.destination.field !== 'prompt') {
+        graph.edges.push({
+          source: edge.source,
+          destination: { node_id: regionalNegativeCondNodeId, field: edge.destination.field },
         });
       }
     }
diff --git a/invokeai/frontend/web/src/features/nodes/util/graph/constants.ts b/invokeai/frontend/web/src/features/nodes/util/graph/constants.ts
index adde745b4a..81952658c8 100644
--- a/invokeai/frontend/web/src/features/nodes/util/graph/constants.ts
+++ b/invokeai/frontend/web/src/features/nodes/util/graph/constants.ts
@@ -47,7 +47,8 @@ export const SDXL_REFINER_INPAINT_CREATE_MASK = 'refiner_inpaint_create_mask';
 export const SEAMLESS = 'seamless';
 export const SDXL_REFINER_SEAMLESS = 'refiner_seamless';
 export const PROMPT_REGION_MASK_PREFIX = 'prompt_region_mask';
-export const PROMPT_REGION_COND_PREFIX = 'prompt_region_cond';
+export const PROMPT_REGION_POSITIVE_COND_PREFIX = 'prompt_region_positive_cond';
+export const PROMPT_REGION_NEGATIVE_COND_PREFIX = 'prompt_region_negative_cond';
 export const POSITIVE_CONDITIONING_COLLECT = 'positive_conditioning_collect';
 export const NEGATIVE_CONDITIONING_COLLECT = 'negative_conditioning_collect';
 
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
index 39a7efd758..b41787ed9f 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
@@ -4,7 +4,8 @@ import { rgbColorToString } from 'features/canvas/util/colorToString';
 import { LayerColorPicker } from 'features/regionalPrompts/components/LayerColorPicker';
 import { LayerMenu } from 'features/regionalPrompts/components/LayerMenu';
 import { LayerVisibilityToggle } from 'features/regionalPrompts/components/LayerVisibilityToggle';
-import { RegionalPromptsPrompt } from 'features/regionalPrompts/components/RegionalPromptsPrompt';
+import { RegionalPromptsNegativePrompt } from 'features/regionalPrompts/components/RegionalPromptsNegativePrompt';
+import { RegionalPromptsPositivePrompt } from 'features/regionalPrompts/components/RegionalPromptsPositivePrompt';
 import { layerSelected } from 'features/regionalPrompts/store/regionalPromptsSlice';
 import { memo, useCallback } from 'react';
 import { useTranslation } from 'react-i18next';
@@ -42,7 +43,8 @@ export const LayerListItem = memo(({ id }: Props) => {
           )}
           <LayerMenu id={id} />
         </Flex>
-        <RegionalPromptsPrompt layerId={id} />
+        <RegionalPromptsPositivePrompt layerId={id} />
+        <RegionalPromptsNegativePrompt layerId={id} />
       </Flex>
     </Flex>
   );
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsNegativePrompt.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsNegativePrompt.tsx
new file mode 100644
index 0000000000..8f5f9f484b
--- /dev/null
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsNegativePrompt.tsx
@@ -0,0 +1,69 @@
+import { Box, Textarea } from '@invoke-ai/ui-library';
+import { useAppDispatch } from 'app/store/storeHooks';
+import { PromptOverlayButtonWrapper } from 'features/parameters/components/Prompts/PromptOverlayButtonWrapper';
+import { AddPromptTriggerButton } from 'features/prompt/AddPromptTriggerButton';
+import { PromptPopover } from 'features/prompt/PromptPopover';
+import { usePrompt } from 'features/prompt/usePrompt';
+import { useLayerNegativePrompt } from 'features/regionalPrompts/hooks/layerStateHooks';
+import { negativePromptChanged } from 'features/regionalPrompts/store/regionalPromptsSlice';
+import { memo, useCallback, useRef } from 'react';
+import type { HotkeyCallback } from 'react-hotkeys-hook';
+import { useHotkeys } from 'react-hotkeys-hook';
+import { useTranslation } from 'react-i18next';
+
+type Props = {
+  layerId: string;
+};
+
+export const RegionalPromptsNegativePrompt = memo((props: Props) => {
+  const prompt = useLayerNegativePrompt(props.layerId);
+  const dispatch = useAppDispatch();
+  const textareaRef = useRef<HTMLTextAreaElement>(null);
+  const { t } = useTranslation();
+  const _onChange = useCallback(
+    (v: string) => {
+      dispatch(negativePromptChanged({ layerId: props.layerId, prompt: v }));
+    },
+    [dispatch, props.layerId]
+  );
+  const { onChange, isOpen, onClose, onOpen, onSelect, onKeyDown, onFocus } = usePrompt({
+    prompt,
+    textareaRef,
+    onChange: _onChange,
+  });
+  const focus: HotkeyCallback = useCallback(
+    (e) => {
+      onFocus();
+      e.preventDefault();
+    },
+    [onFocus]
+  );
+
+  useHotkeys('alt+a', focus, []);
+
+  return (
+    <PromptPopover isOpen={isOpen} onClose={onClose} onSelect={onSelect} width={textareaRef.current?.clientWidth}>
+      <Box pos="relative" w="full">
+        <Textarea
+          id="prompt"
+          name="prompt"
+          ref={textareaRef}
+          value={prompt}
+          placeholder={t('parameters.negativePromptPlaceholder')}
+          onChange={onChange}
+          minH={28}
+          minW={64}
+          onKeyDown={onKeyDown}
+          variant="darkFilled"
+          paddingRight={30}
+          fontSize="sm"
+        />
+        <PromptOverlayButtonWrapper>
+          <AddPromptTriggerButton isOpen={isOpen} onOpen={onOpen} />
+        </PromptOverlayButtonWrapper>
+      </Box>
+    </PromptPopover>
+  );
+});
+
+RegionalPromptsNegativePrompt.displayName = 'RegionalPromptsPrompt';
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPrompt.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPositivePrompt.tsx
similarity index 80%
rename from invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPrompt.tsx
rename to invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPositivePrompt.tsx
index 54fa6df830..4f2e7ca306 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPrompt.tsx
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsPositivePrompt.tsx
@@ -4,8 +4,8 @@ import { PromptOverlayButtonWrapper } from 'features/parameters/components/Promp
 import { AddPromptTriggerButton } from 'features/prompt/AddPromptTriggerButton';
 import { PromptPopover } from 'features/prompt/PromptPopover';
 import { usePrompt } from 'features/prompt/usePrompt';
-import { useLayerPrompt } from 'features/regionalPrompts/hooks/layerStateHooks';
-import { promptChanged } from 'features/regionalPrompts/store/regionalPromptsSlice';
+import { useLayerPositivePrompt } from 'features/regionalPrompts/hooks/layerStateHooks';
+import { positivePromptChanged } from 'features/regionalPrompts/store/regionalPromptsSlice';
 import { memo, useCallback, useRef } from 'react';
 import type { HotkeyCallback } from 'react-hotkeys-hook';
 import { useHotkeys } from 'react-hotkeys-hook';
@@ -15,14 +15,14 @@ type Props = {
   layerId: string;
 };
 
-export const RegionalPromptsPrompt = memo((props: Props) => {
-  const prompt = useLayerPrompt(props.layerId);
+export const RegionalPromptsPositivePrompt = memo((props: Props) => {
+  const prompt = useLayerPositivePrompt(props.layerId);
   const dispatch = useAppDispatch();
   const textareaRef = useRef<HTMLTextAreaElement>(null);
   const { t } = useTranslation();
   const _onChange = useCallback(
     (v: string) => {
-      dispatch(promptChanged({ layerId: props.layerId, prompt: v }));
+      dispatch(positivePromptChanged({ layerId: props.layerId, prompt: v }));
     },
     [dispatch, props.layerId]
   );
@@ -65,4 +65,4 @@ export const RegionalPromptsPrompt = memo((props: Props) => {
   );
 });
 
-RegionalPromptsPrompt.displayName = 'RegionalPromptsPrompt';
+RegionalPromptsPositivePrompt.displayName = 'RegionalPromptsPrompt';
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/hooks/layerStateHooks.ts b/invokeai/frontend/web/src/features/regionalPrompts/hooks/layerStateHooks.ts
index 067c0b71d6..0b4729dcbb 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/hooks/layerStateHooks.ts
+++ b/invokeai/frontend/web/src/features/regionalPrompts/hooks/layerStateHooks.ts
@@ -17,12 +17,26 @@ export const useLayer = (layerId: string) => {
   return layer;
 };
 
-export const useLayerPrompt = (layerId: string) => {
+export const useLayerPositivePrompt = (layerId: string) => {
   const selectLayer = useMemo(
     () =>
       createSelector(
         selectRegionalPromptsSlice,
-        (regionalPrompts) => regionalPrompts.layers.find((l) => l.id === layerId)?.prompt
+        (regionalPrompts) => regionalPrompts.layers.find((l) => l.id === layerId)?.positivePrompt
+      ),
+    [layerId]
+  );
+  const prompt = useAppSelector(selectLayer);
+  assert(prompt !== undefined, `Layer ${layerId} doesn't exist!`);
+  return prompt;
+};
+
+export const useLayerNegativePrompt = (layerId: string) => {
+  const selectLayer = useMemo(
+    () =>
+      createSelector(
+        selectRegionalPromptsSlice,
+        (regionalPrompts) => regionalPrompts.layers.find((l) => l.id === layerId)?.negativePrompt
       ),
     [layerId]
   );
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts b/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
index f760ec9e91..28f941e642 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
+++ b/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
@@ -52,7 +52,8 @@ type LayerBase = {
 type PromptRegionLayer = LayerBase & {
   kind: 'promptRegionLayer';
   objects: LayerObject[];
-  prompt: string;
+  positivePrompt: string;
+  negativePrompt: string;
   color: RgbColor;
 };
 
@@ -73,7 +74,7 @@ const initialRegionalPromptsState: RegionalPromptsState = {
   selectedLayer: null,
   brushSize: 40,
   layers: [],
-  promptLayerOpacity: 0.5,
+  promptLayerOpacity: 0.5, // This currently doesn't work
 };
 
 const isLine = (obj: LayerObject): obj is LineObject => obj.kind === 'line';
@@ -89,7 +90,8 @@ export const regionalPromptsSlice = createSlice({
           isVisible: true,
           bbox: null,
           kind: action.payload,
-          prompt: '',
+          positivePrompt: '',
+          negativePrompt: '',
           objects: [],
           color: action.meta.color,
           x: 0,
@@ -118,7 +120,6 @@ export const regionalPromptsSlice = createSlice({
       layer.objects = [];
       layer.bbox = null;
       layer.isVisible = true;
-      layer.prompt = '';
     },
     layerDeleted: (state, action: PayloadAction<string>) => {
       state.layers = state.layers.filter((l) => l.id !== action.payload);
@@ -163,13 +164,21 @@ export const regionalPromptsSlice = createSlice({
       state.layers = [];
       state.selectedLayer = null;
     },
-    promptChanged: (state, action: PayloadAction<{ layerId: string; prompt: string }>) => {
+    positivePromptChanged: (state, action: PayloadAction<{ layerId: string; prompt: string }>) => {
       const { layerId, prompt } = action.payload;
       const layer = state.layers.find((l) => l.id === layerId);
       if (!layer) {
         return;
       }
-      layer.prompt = prompt;
+      layer.positivePrompt = prompt;
+    },
+    negativePromptChanged: (state, action: PayloadAction<{ layerId: string; prompt: string }>) => {
+      const { layerId, prompt } = action.payload;
+      const layer = state.layers.find((l) => l.id === layerId);
+      if (!layer) {
+        return;
+      }
+      layer.negativePrompt = prompt;
     },
     promptRegionLayerColorChanged: (state, action: PayloadAction<{ layerId: string; color: RgbColor }>) => {
       const { layerId, color } = action.payload;
@@ -254,7 +263,8 @@ export const {
   layerReset,
   layerDeleted,
   layerIsVisibleToggled,
-  promptChanged,
+  positivePromptChanged,
+  negativePromptChanged,
   lineAdded,
   pointsAdded,
   promptRegionLayerColorChanged,
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/util/getLayerBlobs.ts b/invokeai/frontend/web/src/features/regionalPrompts/util/getLayerBlobs.ts
index ce6f751479..52bca435cf 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/util/getLayerBlobs.ts
+++ b/invokeai/frontend/web/src/features/regionalPrompts/util/getLayerBlobs.ts
@@ -48,7 +48,7 @@ export const getRegionalPromptLayerBlobs = async (
 
     if (preview) {
       const base64 = await blobToDataURL(blob);
-      openBase64ImageInTab([{ base64, caption: `${reduxLayer.id}: ${reduxLayer.prompt}` }]);
+      openBase64ImageInTab([{ base64, caption: `${reduxLayer.id}: ${reduxLayer.positivePrompt} / ${reduxLayer.negativePrompt}` }]);
     }
     layer.remove();
     blobs[layer.id()] = blob;