diff --git a/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts b/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
index 03a042a6cf..dd3f40da85 100644
--- a/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
+++ b/invokeai/frontend/web/src/features/nodes/util/graph/addRegionalPromptsToGraph.ts
@@ -22,7 +22,6 @@ export const addRegionalPromptsToGraph = async (state: RootState, graph: NonNull
   const { dispatch } = getStore();
   // TODO: Handle non-SDXL
   // const isSDXL = state.generation.model?.base === 'sdxl';
-  const { autoNegative } = state.regionalPrompts.present;
   const layers = state.regionalPrompts.present.layers
     .filter((l) => l.kind === 'promptRegionLayer') // We only want the prompt region layers
     .filter((l) => l.isVisible); // Only visible layers are rendered on the canvas
@@ -205,7 +204,7 @@ export const addRegionalPromptsToGraph = async (state: RootState, graph: NonNull
     }
 
     // If we are using the "invert" auto-negative setting, we need to add an additional negative conditioning node
-    if (autoNegative === 'invert') {
+    if (layer.autoNegative === 'invert') {
       // We re-use the mask image, but invert it when converting to tensor
       // TODO: Probably faster to invert the tensor from the earlier mask rather than read the mask image and convert...
       const invertedMaskToTensorNode: S['AlphaMaskToTensorInvocation'] = {
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/LayerAutoNegativeCombobox.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerAutoNegativeCombobox.tsx
new file mode 100644
index 0000000000..0f29b41287
--- /dev/null
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerAutoNegativeCombobox.tsx
@@ -0,0 +1,62 @@
+import type { ComboboxOnChange, ComboboxOption } from '@invoke-ai/ui-library';
+import { Combobox, FormControl, FormLabel } from '@invoke-ai/ui-library';
+import { createSelector } from '@reduxjs/toolkit';
+import { useAppDispatch, useAppSelector } from 'app/store/storeHooks';
+import { isParameterAutoNegative } from 'features/parameters/types/parameterSchemas';
+import {
+  layerAutoNegativeChanged,
+  selectRegionalPromptsSlice,
+} from 'features/regionalPrompts/store/regionalPromptsSlice';
+import { memo, useCallback, useMemo } from 'react';
+import { useTranslation } from 'react-i18next';
+import { assert } from 'tsafe';
+
+const options: ComboboxOption[] = [
+  { label: 'Off', value: 'off' },
+  { label: 'Invert', value: 'invert' },
+];
+
+type Props = {
+  layerId: string;
+};
+
+const useAutoNegative = (layerId: string) => {
+  const selectAutoNegative = useMemo(
+    () =>
+      createSelector(selectRegionalPromptsSlice, (regionalPrompts) => {
+        const layer = regionalPrompts.present.layers.find((l) => l.id === layerId);
+        assert(layer, `Layer ${layerId} not found`);
+        return layer.autoNegative;
+      }),
+    [layerId]
+  );
+  const autoNegative = useAppSelector(selectAutoNegative);
+  return autoNegative;
+};
+
+const AutoNegativeCombobox = ({ layerId }: Props) => {
+  const dispatch = useAppDispatch();
+  const { t } = useTranslation();
+  const autoNegative = useAutoNegative(layerId);
+
+  const onChange = useCallback<ComboboxOnChange>(
+    (v) => {
+      if (!isParameterAutoNegative(v?.value)) {
+        return;
+      }
+      dispatch(layerAutoNegativeChanged({ layerId, autoNegative: v.value }));
+    },
+    [dispatch, layerId]
+  );
+
+  const value = useMemo(() => options.find((o) => o.value === autoNegative), [autoNegative]);
+
+  return (
+    <FormControl flexGrow={0} gap={2} w="min-content">
+      <FormLabel m={0}>AutoNegative</FormLabel>
+      <Combobox value={value} options={options} onChange={onChange} isSearchable={false} sx={{ w: '5.2rem' }} />
+    </FormControl>
+  );
+};
+
+export default memo(AutoNegativeCombobox);
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
index 0d84885010..e9ae02bd34 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/LayerListItem.tsx
@@ -1,6 +1,7 @@
-import { Flex, Spacer, Text } from '@invoke-ai/ui-library';
+import { Flex, Spacer } from '@invoke-ai/ui-library';
 import { useAppDispatch, useAppSelector } from 'app/store/storeHooks';
 import { rgbaColorToString } from 'features/canvas/util/colorToString';
+import LayerAutoNegativeCombobox from 'features/regionalPrompts/components/LayerAutoNegativeCombobox';
 import { LayerColorPicker } from 'features/regionalPrompts/components/LayerColorPicker';
 import { LayerMenu } from 'features/regionalPrompts/components/LayerMenu';
 import { LayerVisibilityToggle } from 'features/regionalPrompts/components/LayerVisibilityToggle';
@@ -8,7 +9,6 @@ import { RegionalPromptsNegativePrompt } from 'features/regionalPrompts/componen
 import { RegionalPromptsPositivePrompt } from 'features/regionalPrompts/components/RegionalPromptsPositivePrompt';
 import { layerSelected } from 'features/regionalPrompts/store/regionalPromptsSlice';
 import { memo, useCallback } from 'react';
-import { useTranslation } from 'react-i18next';
 
 type Props = {
   id: string;
@@ -16,7 +16,6 @@ type Props = {
 
 export const LayerListItem = memo(({ id }: Props) => {
   const dispatch = useAppDispatch();
-  const { t } = useTranslation();
   const selectedLayer = useAppSelector((s) => s.regionalPrompts.present.selectedLayer);
   const color = useAppSelector((s) => {
     const color = s.regionalPrompts.present.layers.find((l) => l.id === id)?.color;
@@ -36,11 +35,7 @@ export const LayerListItem = memo(({ id }: Props) => {
           <LayerColorPicker id={id} />
           <LayerVisibilityToggle id={id} />
           <Spacer />
-          {selectedLayer === id && (
-            <Text color="base.300" fontWeight="semibold" pe={2}>
-              {t('common.selected')}
-            </Text>
-          )}
+          <LayerAutoNegativeCombobox layerId={id} />
           <LayerMenu id={id} />
         </Flex>
         <RegionalPromptsPositivePrompt layerId={id} />
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/NegativeModeCombobox.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/NegativeModeCombobox.tsx
deleted file mode 100644
index 8c818ff930..0000000000
--- a/invokeai/frontend/web/src/features/regionalPrompts/components/NegativeModeCombobox.tsx
+++ /dev/null
@@ -1,39 +0,0 @@
-import type { ComboboxOnChange, ComboboxOption } from '@invoke-ai/ui-library';
-import { Combobox, FormControl, FormLabel } from '@invoke-ai/ui-library';
-import { useAppDispatch, useAppSelector } from 'app/store/storeHooks';
-import { isParameterAutoNegative } from 'features/parameters/types/parameterSchemas';
-import { autoNegativeChanged } from 'features/regionalPrompts/store/regionalPromptsSlice';
-import { memo, useCallback, useMemo } from 'react';
-import { useTranslation } from 'react-i18next';
-
-const options: ComboboxOption[] = [
-  { label: 'Off', value: 'off' },
-  { label: 'Invert', value: 'invert' },
-];
-
-const AutoNegativeCombobox = () => {
-  const dispatch = useAppDispatch();
-  const { t } = useTranslation();
-  const autoNegative = useAppSelector((s) => s.regionalPrompts.present.autoNegative);
-
-  const onChange = useCallback<ComboboxOnChange>(
-    (v) => {
-      if (!isParameterAutoNegative(v?.value)) {
-        return;
-      }
-      dispatch(autoNegativeChanged(v.value));
-    },
-    [dispatch]
-  );
-
-  const value = useMemo(() => options.find((o) => o.value === autoNegative), [autoNegative]);
-
-  return (
-    <FormControl>
-      <FormLabel>Negative Mode</FormLabel>
-      <Combobox value={value} options={options} onChange={onChange} isSearchable={false} />
-    </FormControl>
-  );
-};
-
-export default memo(AutoNegativeCombobox);
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsEditor.tsx b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsEditor.tsx
index 26a10c4526..02e10f6b8c 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsEditor.tsx
+++ b/invokeai/frontend/web/src/features/regionalPrompts/components/RegionalPromptsEditor.tsx
@@ -6,7 +6,6 @@ import { AddLayerButton } from 'features/regionalPrompts/components/AddLayerButt
 import { BrushSize } from 'features/regionalPrompts/components/BrushSize';
 import { DeleteAllLayersButton } from 'features/regionalPrompts/components/DeleteAllLayersButton';
 import { LayerListItem } from 'features/regionalPrompts/components/LayerListItem';
-import AutoNegativeCombobox from 'features/regionalPrompts/components/NegativeModeCombobox';
 import { PromptLayerOpacity } from 'features/regionalPrompts/components/PromptLayerOpacity';
 import { StageComponent } from 'features/regionalPrompts/components/StageComponent';
 import { ToolChooser } from 'features/regionalPrompts/components/ToolChooser';
@@ -39,7 +38,6 @@ export const RegionalPromptsEditor = memo(() => {
         </Flex>
         <BrushSize />
         <PromptLayerOpacity />
-        <AutoNegativeCombobox />
         {layerIdsReversed.map((id) => (
           <LayerListItem key={id} id={id} />
         ))}
diff --git a/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts b/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
index ad487d83e8..5612d093e3 100644
--- a/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
+++ b/invokeai/frontend/web/src/features/regionalPrompts/store/regionalPromptsSlice.ts
@@ -56,6 +56,7 @@ type PromptRegionLayer = LayerBase & {
   positivePrompt: string;
   negativePrompt: string;
   color: RgbColor;
+  autoNegative: ParameterAutoNegative;
 };
 
 export type Layer = PromptRegionLayer;
@@ -67,7 +68,6 @@ type RegionalPromptsState = {
   layers: PromptRegionLayer[];
   brushSize: number;
   promptLayerOpacity: number;
-  autoNegative: ParameterAutoNegative;
 };
 
 export const initialRegionalPromptsState: RegionalPromptsState = {
@@ -77,7 +77,6 @@ export const initialRegionalPromptsState: RegionalPromptsState = {
   brushSize: 40,
   layers: [],
   promptLayerOpacity: 0.5, // This currently doesn't work
-  autoNegative: 'off',
 };
 
 const isLine = (obj: LayerObject): obj is LineObject => obj.kind === 'line';
@@ -99,6 +98,7 @@ export const regionalPromptsSlice = createSlice({
           color: action.meta.color,
           x: 0,
           y: 0,
+          autoNegative: 'off',
         };
         state.layers.push(layer);
         state.selectedLayer = layer.id;
@@ -233,8 +233,16 @@ export const regionalPromptsSlice = createSlice({
     promptLayerOpacityChanged: (state, action: PayloadAction<number>) => {
       state.promptLayerOpacity = action.payload;
     },
-    autoNegativeChanged: (state, action: PayloadAction<ParameterAutoNegative>) => {
-      state.autoNegative = action.payload;
+    layerAutoNegativeChanged: (
+      state,
+      action: PayloadAction<{ layerId: string; autoNegative: ParameterAutoNegative }>
+    ) => {
+      const { layerId, autoNegative } = action.payload;
+      const layer = state.layers.find((l) => l.id === layerId);
+      if (!layer || layer.kind !== 'promptRegionLayer') {
+        return;
+      }
+      layer.autoNegative = autoNegative;
     },
     lineFinished: (state) => {
       console.log('lineFinished');
@@ -288,7 +296,7 @@ export const {
   layerBboxChanged,
   promptLayerOpacityChanged,
   allLayersDeleted,
-  autoNegativeChanged,
+  layerAutoNegativeChanged,
 } = regionalPromptsSlice.actions;
 
 export const selectRegionalPromptsSlice = (state: RootState) => state.regionalPrompts;